APX1-pm-eGFP
(Plasmid
#245520)
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PurposePlasmid for PiggyBac /ITR mediated insertion of pm-eGFP downstream of CAG promoter using Gateway recombination cassette
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Depositing Labs
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 245520 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 |
Backbone
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Vector backboneAPX1
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Backbone manufacturerTimothy Sanders
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namepm-eGFP
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Alt nameGAP-43 N-terminus
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SpeciesR. norvegicus (rat)
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Insert Size (bp)800
Cloning Information
- Cloning method Gateway Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
APX1-pm-eGFP was a gift from Timothy Sanders & Kate Storey (Addgene plasmid # 245520 ; http://n2t.net/addgene:245520 ; RRID:Addgene_245520) -
For your References section:
Engineering fluorescent reporters in human pluripotent stem cells and strategies for live imaging human neurogenesis. Dady A, Davidson L, Loyer N, Rappich S, Findlay G, Sanders T, Januschke J, Storey KG. Development. 2025 Aug 1:dev.205082. doi: 10.1242/dev.205082. 10.1242/dev.205082 PubMed 40748212