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bRA66-Mic60chimeras
(Plasmid #245525)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 245525 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    bRA66
  • Backbone manufacturer
    Plasmid #100952, Anand et al. 2017
  • Backbone size w/o insert (bp) 10931
  • Total vector size (bp) 10951
  • Modifications to backbone
    gRNA to create S. cerevisiae Mic60 chimeras
  • Vector type
    CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    YKR016w
  • gRNA/shRNA sequence
    CTACGCTTAATAGCGATGGA
  • Species
    S. cerevisiae (budding yeast)
  • GenBank ID
    Z28241.1 Z28241.1
  • Entrez Gene
    MIC60 (a.k.a. YKR016W, AIM28, FCJ1, FMP13)
  • Promoter T7 TAATACGACTCACTATAGG

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer GTAAAACGACGGCCAGT
  • 3′ sequencing primer GTCATAGCTGTTTCCTG
  • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    bRA66 was obtained from Addgene from James Haber (Addgene plasmid # 100952 ; http://n2t.net/addgene:100952 ; RRID:Addgene_100952; Anand et al., 2017)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    bRA66-Mic60chimeras was a gift from Luke Chao (Addgene plasmid # 245525 ; http://n2t.net/addgene:245525 ; RRID:Addgene_245525)

  • For your References section:

    Ancestral sequence reconstruction of the Mic60 Mitofilin domain reveals residues supporting respiration in yeast. Benning FMC, Bell TA, Nguyen TH, Syau D, Connell LB, Liao YT, Keating MP, Coughlin M, Nordstrom AEH, Ericsson M, daCosta CJB, Chao LH. Protein Sci. 2025 Jul;34(7):e70207. doi: 10.1002/pro.70207. 10.1002/pro.70207 PubMed 40545685
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