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pICSL12047
(Plasmid #245644)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 245644 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pICH41295
  • Backbone size w/o insert (bp) 2249
  • Vector type
    Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SGT-34S FMV (Figwort Mosaic Virus). Sub-Genomic Transcript (SGT) promoter
  • Species
    Caulimovirus
  • Insert Size (bp)
    364
  • Mutation
    BsaI/BpiI sites removed by point-mutation

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BsaI (destroyed during cloning)
  • 3′ cloning site BsaI (destroyed during cloning)
  • 5′ sequencing primer TCACATGTTCTTTCCTGCG
  • 3′ sequencing primer GTCTCATGAGCGGATACATATTTGAATG
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Level 0 promoter + 5' untranslated region module used for GoldenGate assemblies using the MoClo overhang syntax

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pICSL12047 was a gift from Mark Youles (Addgene plasmid # 245644 ; http://n2t.net/addgene:245644 ; RRID:Addgene_245644)
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