pICSL41308_CP
(Plasmid
#245695)
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Purpose(Empty Backbone) Level 0 Golden Gate Coding Sequence (WITH stop codon) acceptor with AmilCP negative selection cassette AATG - GCTT
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 245695 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepICH41308
- Backbone size (bp) 2247
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Vector typeSynthetic Biology
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Selectable markersAmil CP purple chromoprotein for purple/white negative selection
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNone
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MutationBsaI/BpiI sites removed by point-mutation
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Level 0 Acceptor for making coding sequence modules used for GoldenGate assemblies using the MoClo overhang syntax. Plant Golden-gate Promoters, Terminators and Level0/Level1 cloning acceptors. Insert Size: 1106, Species: Synthetic. 5' Cloning Site: BpiI (Destroyed), 3' Cloning Site: BpiI (Destroyed).
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pICSL41308_CP was a gift from Mark Youles (Addgene plasmid # 245695 ; http://n2t.net/addgene:245695 ; RRID:Addgene_245695)