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pICSL41308-LC
(Plasmid #245696)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 245696 Standard format: Plasmid sent in bacteria as agar stab 1 $89

Backbone

  • Vector backbone
    pICH41308
  • Backbone size (bp) 3495
  • Vector type
    Synthetic Biology
  • Selectable markers
    Lac Z for blue/white negative selction with the addition of X-gal and ITPG

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    None
  • Mutation
    BsaI/BpiI sites removed by point-mutation

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Level 0 Acceptor for making low-copy coding sequence modules used for GoldenGate assemblies using the MoClo overhang syntax. Plant Golden-gate Promoters, Terminators and Level0/Level1 cloning acceptors. Insert Size: 598, Species: Synthetic. 5' Cloning Site: BpiI (Destroyed), 3' Cloning Site: BpiI (Destroyed).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pICSL41308-LC was a gift from Mark Youles (Addgene plasmid # 245696 ; http://n2t.net/addgene:245696 ; RRID:Addgene_245696)