pICSL41308-LC
(Plasmid
#245696)
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Purpose(Empty Backbone) Level 0 Golden Gate Coding Sequence (WITH stop codon) acceptor, Low - copy with pBR322 origin of replication AATG - GCTT
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 245696 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepICH41308
- Backbone size (bp) 3495
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Vector typeSynthetic Biology
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Selectable markersLac Z for blue/white negative selction with the addition of X-gal and ITPG
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameNone
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MutationBsaI/BpiI sites removed by point-mutation
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Level 0 Acceptor for making low-copy coding sequence modules used for GoldenGate assemblies using the MoClo overhang syntax. Plant Golden-gate Promoters, Terminators and Level0/Level1 cloning acceptors. Insert Size: 598, Species: Synthetic. 5' Cloning Site: BpiI (Destroyed), 3' Cloning Site: BpiI (Destroyed).
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pICSL41308-LC was a gift from Mark Youles (Addgene plasmid # 245696 ; http://n2t.net/addgene:245696 ; RRID:Addgene_245696)