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pICSL47742_RFP
(Plasmid #245699)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 245699 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pICH47742
  • Backbone size (bp) 4372
  • Vector type
    Synthetic Biology
  • Selectable markers
    Amil RFP red chromoprotein for red/white negative selection

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    None
  • Mutation
    BsaI/BpiI sites removed by point-mutation

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Level 1 Acceptor for making full transcriptional unit modules in Level 1 position 2 (FORWARD) used for GoldenGate assemblies using the MoClo overhang syntax. Plant Golden-gate Promoters, Terminators and Level0/Level1 cloning acceptors. Insert Size: 1092, Species: Synthetic. 5' Cloning Site: BsaI (Destroyed), 3' Cloning Site: BsaI (Destroyed).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pICSL47742_RFP was a gift from Mark Youles (Addgene plasmid # 245699 ; http://n2t.net/addgene:245699 ; RRID:Addgene_245699)
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