pBD24
(Plasmid
#245764)
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PurposeAllows for rap-1::mCherry entry into Gateway Vectors
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 245764 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 |
Backbone
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Vector backbonepCR8/GW/TOPO
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Vector typeUnspecified
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namerap-1::mCherry
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SpeciesC. elegans (nematode)
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Tag
/ Fusion Protein
- mCherry (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBD24 was a gift from Salvatore Cherra (Addgene plasmid # 245764) -
For your References section:
A PDZ-RapGEF promotes synaptic development in C. elegans through a Rap/Rac signaling pathway. Lamb R, Scales M, Watkins J, Werner M, Cherra SJ. Development. 2025 Aug 1:dev.204678. doi: 10.1242/dev.204678. 10.1242/dev.204678 PubMed 40748210