pBD34
(Plasmid
#245769)
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PurposeExpresses tiam-1::mGFP in cholinergic motor neurons
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 245769 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 |
Backbone
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Vector backbonepDEST
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Vector typeWorm Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nametiam-1::mGFP
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SpeciesC. elegans (nematode)
- Promoter unc-17b
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Tag
/ Fusion Protein
- mGFP (C terminal on insert)
Cloning Information
- Cloning method Gateway Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBD34 was a gift from Salvatore Cherra (Addgene plasmid # 245769) -
For your References section:
A PDZ-RapGEF promotes synaptic development in C. elegans through a Rap/Rac signaling pathway. Lamb R, Scales M, Watkins J, Werner M, Cherra SJ. Development. 2025 Aug 1:dev.204678. doi: 10.1242/dev.204678. 10.1242/dev.204678 PubMed 40748210