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Addgene

pREA12
(Plasmid #245770)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 245770 Standard format: Plasmid sent in bacteria as agar stab 1 $89

Backbone

  • Vector backbone
    pCR8/GW/TOPO
  • Vector type
    Unspecified

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mCherry::RBD::T2A::rac-2::mGFP
  • Species
    C. elegans (nematode)
  • Tags / Fusion Proteins
    • mCherry (N terminal on insert)
    • mGFP (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pREA12 was a gift from Salvatore Cherra (Addgene plasmid # 245770)

  • For your References section:

    A PDZ-RapGEF promotes synaptic development in C. elegans through a Rap/Rac signaling pathway. Lamb R, Scales M, Watkins J, Werner M, Cherra SJ. Development. 2025 Aug 1:dev.204678. doi: 10.1242/dev.204678. 10.1242/dev.204678 PubMed 40748210
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