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pSmBtA3-d7F
(Plasmid #245784)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 245784 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pcDNA3
  • Backbone size w/o insert (bp) 5400
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    arrestin-3
  • Species
    B. taurus (bovine)
  • Insert Size (bp)
    1400
  • Mutation
    deleted residues 175, 177, 178, 182-185
  • Entrez Gene
    ARR3
  • Promoter CMV
  • Tags / Fusion Proteins
    • SmBiT (N terminal on insert)
    • Flag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (unknown if destroyed)
  • 3′ cloning site HindIII (unknown if destroyed)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSmBtA3-d7F was a gift from Vsevolod Gurevich (Addgene plasmid # 245784 ; http://n2t.net/addgene:245784 ; RRID:Addgene_245784)

  • For your References section:

    GPCR Binding and JNK3 Activation by Arrestin-3 Have Different Structural Requirements. Zheng C, Weinstein LD, Nguyen KK, Grewal A, Gurevich EV, Gurevich VV. Cells. 2023 Jun 6;12(12):1563. doi: 10.3390/cells12121563. 10.3390/cells12121563 PubMed 37371033
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