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AAV CAG-FLEx-Rpl22-3xHA
(Plasmid #245798)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 245798 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pAAV-CAG-FLEX
  • Backbone manufacturer
    Ryohei Yasuda (Addgene #84357)
  • Backbone size w/o insert (bp) 5006
  • Total vector size (bp) 5495
  • Vector type
    AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Ribosomal protein L22
  • Alt name
    Rpl22
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    489
  • GenBank ID
    NM_001277113.1
  • Entrez Gene
    Rpl22 (a.k.a. 2700038K18Rik)
  • Promoter CAG
  • Tag / Fusion Protein
    • 3xHA (C terminal on insert)

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer GCAACGTGCTGGTTATTGTG
  • 3′ sequencing primer CATAGCGTAAAAGGAGCAACA
    • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Khakh Lab, Addgene #111811

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    AAV CAG-FLEx-Rpl22-3xHA was a gift from Baljit Khakh (Addgene plasmid # 245798 ; http://n2t.net/addgene:245798 ; RRID:Addgene_245798)
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