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TAPBPR S104F K211L R270Q
(Plasmid #245834)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 245834 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pMT_puro
  • Backbone size w/o insert (bp) 4724
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    TAPBPR (S104F K211L R270Q)
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1329
  • Mutation
    S104F K211L R270Q (HiFi mutants) & C94S (removed free cysteine)
  • Entrez Gene
    TAPBPL (a.k.a. TAPBP-R, TAPBPR)
  • Promoter MT_puro
  • Tags / Fusion Proteins
    • AviTag biotinylation tag
    • His6 affinity tag

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    TAPBPR S104F K211L R270Q was a gift from Nikolaos Sgourakis (Addgene plasmid # 245834 ; http://n2t.net/addgene:245834 ; RRID:Addgene_245834)

  • For your References section:

    Xeno interactions between MHC-I proteins and molecular chaperones enable ligand exchange on a broad repertoire of HLA allotypes. Sun Y, Papadaki GF, Devlin CA, Danon JN, Young MC, Winters TJ, Burslem GM, Procko E, Sgourakis NG. Sci Adv. 2023 Feb 24;9(8):eade7151. doi: 10.1126/sciadv.ade7151. Epub 2023 Feb 24. 10.1126/sciadv.ade7151 PubMed 36827371
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