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pDR-pBAD-ATG-6xHIS-AsiA
(Plasmid #245835)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 245835 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pBAD
  • Backbone size w/o insert (bp) 5284
  • Total vector size (bp) 5572
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    AsiA
  • Species
    T4 Bacteriophage
  • Insert Size (bp)
    288
  • Promoter araBAD
  • Tag / Fusion Protein
    • 6xHis (N terminal on insert)

Cloning Information

  • Cloning method Other
  • 5′ sequencing primer GAGATATACGCTAGC ATGCATCATCATCATCATCAT AACAAAAACATTGATACCGTGCGT
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDR-pBAD-ATG-6xHIS-AsiA was a gift from Jie Xiao (Addgene plasmid # 245835 ; http://n2t.net/addgene:245835 ; RRID:Addgene_245835)

  • For your References section:

    Spatial organization of RNA polymerase and its relationship with transcription in Escherichia coli. Weng X, Bohrer CH, Bettridge K, Lagda AC, Cagliero C, Jin DJ, Xiao J. Proc Natl Acad Sci U S A. 2019 Oct 1;116(40):20115-20123. doi: 10.1073/pnas.1903968116. Epub 2019 Sep 16. 10.1073/pnas.1903968116 PubMed 31527272
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