LCB70
              
              
                (Plasmid
                
                #245856)
              
            
            
            
          - 
            PurposeArchael (Methanosuratincola verstraetei LCB70) 16S rRNA expression
- 
              Depositing Lab
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          Publication
- 
          Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 245856 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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            Vector backbonepcr2.1-oriT
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              Vector typeOther
Growth in Bacteria
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            Bacterial Resistance(s)Ampicillin and Kanamycin, 100 & 50 μg/mL
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            Growth Temperature37°C
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            Growth Strain(s)DH5alpha
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            Copy numberHigh Copy
Gene/Insert
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                Gene/Insert name16S rRNA
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                    SpeciesMethanosuratincola verstraetei LCB70
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer ATTGTAATACGACTCACTATAGGGCGAATT
- 3′ sequencing primer CTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGTGCATGCTCGAGCGGCCGCCAGTGTGATGG (Common Sequencing Primers)
Resource Information
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            Supplemental Documents
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            A portion of this plasmid was derived from a plasmid made byDOE Joint Genome Institute
Terms and Licenses
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        Academic/Nonprofit Terms
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      Industry Terms- Not Available to Industry
 
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
- 
              For your Materials & Methods section: LCB70 was a gift from Roland Hatzenpichler (Addgene plasmid # 245856 ; http://n2t.net/addgene:245856 ; RRID:Addgene_245856)
 
    
 
    
