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(Plasmid #24590)

Full plasmid sequence is not available for this item.



Item Catalog # Description Quantity Price (USD)
Plasmid 24590 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Ampicillin, 25 & 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
    ccdB Survival
  • Growth instructions
    DB3.1 or ccdB resistance strain. We do not allow E. coli harbouring this plasmid to grow to saturation (no longer than 14 hours). We prefer to grow it at 30C and 180RPM of shaking when applicable.
  • Copy number
    Low Copy


  • Gene/Insert name
    Gateway(TM) cassette
  • Insert Size (bp)
  • Tag / Fusion Protein
    • VM (N terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

pLD-hygro-EnVM was constructed by subcloning the hygromycin resistance gene from the pLJM6 vector (J Moffat, unpublished) into the pLD-puro-EnFH vector using SpeI/NsiI and subsequently subcloning a gene synthesized V5-Myc (VM) tag (Bio Basic Inc.) from the pUC57 host vector.

pLD-puro-EnFH (pLD-puromycin resistance- EF1alpha, N-terminus triple Flag-6-His) was generated from pLKO.1 (see paper for details).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLD-hygro-EnVM was a gift from Jason Moffat (Addgene plasmid # 24590 ; ; RRID:Addgene_24590)
  • For your References section:

    A lentiviral-based functional proteomics approach identifies chromatin remodelling complexes important for the induction of pluripotency. Mak AB, Ni Z, Hewel JA, Chen GI, Zhong G, Karamboulas K, Blakely K, Smiley S, Marcon E, Roudeva D, Li J, Olsen JB, Punna T, Isserlin R, Chetyrkin S, Gingras AC, Emili A, Greenblatt J, Moffat J. Mol Cell Proteomics. 2010 May;9(5):811-23. 10.1074/mcp.M000002-MCP201 PubMed 20305087