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GFP-CDKL5(isoform1)_L960D
(Plasmid #245900)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 245900 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pEGFP-C1
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CDKL5-2
  • Species
    H. sapiens (human)
  • Mutation
    N8D, E569G, L960D
  • Entrez Gene
    CDKL5 (a.k.a. CFAP247, DEE2, EIEE2, ISSX, STK9)
  • Promoter CMV
  • Tag / Fusion Protein
    • GFP-Avitag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer EGFP-C
  • 3′ sequencing primer SV40pA-R
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2025.08.27.672337 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GFP-CDKL5(isoform1)_L960D was a gift from Ben Goult (Addgene plasmid # 245900 ; http://n2t.net/addgene:245900 ; RRID:Addgene_245900)

  • For your References section:

    Cyclin-dependent kinase-like 5 (CDKL5) binds to talin and is anchored at the postsynaptic density via direct interaction with PDZ domains. Otani Y, Goodall E, Srinivasan V, Ball NJ, Barnett SFH, Zech T, Saarikangas J, Goult BT. bioRxiv 2025.08.27.672337; doi: https://doi.org/10.1101/2025.08.27.672337 10.1101/2025.08.27.672337
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