mSHANK2_PDZ
(Plasmid
#245907)
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PurposeBacterial expression of SHANK2 PDZ domain (247-340)
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 245907 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepET151 dTOPO
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSHANK2
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SpeciesM. musculus (mouse)
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Entrez GeneShank2 (a.k.a. ProSAP1, mKIAA1022)
- Promoter T7
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Tag
/ Fusion Protein
- HisTag-V5 (N terminal on insert)
Cloning Information
- Cloning method Gene Synthesis
- 5′ sequencing primer T7F
- 3′ sequencing primer T7R (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
mSHANK2_PDZ was a gift from Ben Goult (Addgene plasmid # 245907 ; http://n2t.net/addgene:245907 ; RRID:Addgene_245907) -
For your References section:
Cyclin-dependent kinase-like 5 (CDKL5) binds to talin and is anchored at the postsynaptic density via direct interaction with PDZ domains. Otani Y, Goodall E, Srinivasan V, Ball NJ, Barnett SFH, Zech T, Saarikangas J, Goult BT. bioRxiv 2025.08.27.672337; doi: https://doi.org/10.1101/2025.08.27.672337 10.1101/2025.08.27.672337