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CMV-huADF WT
(Plasmid #245941)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 245941 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone manufacturer
    Unknown
  • Backbone size w/o insert (bp) 5428
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    actin depolymerizing factor (ADF)
  • Alt name
    destrin
  • Species
    H. sapiens (human)
  • Entrez Gene
    DSTN (a.k.a. ACTDP, ADF, HEL32, bA462D18.2)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unspecified (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Human ADF is not targeted by cofilin shRNA

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CMV-huADF WT was a gift from James Bamburg & Alan Weeds (Addgene plasmid # 245941 ; http://n2t.net/addgene:245941 ; RRID:Addgene_245941)

  • For your References section:

    Human actin depolymerizing factor mediates a pH-sensitive destruction of actin filaments. Hawkins M, Pope B, Maciver SK, Weeds AG. Biochemistry. 1993 Sep 28;32(38):9985-93. doi: 10.1021/bi00089a014. 10.1021/bi00089a014 PubMed 8399167
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