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XAC1 S3E
(Plasmid #245947)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 245947 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone manufacturer
    Unknown
  • Backbone size w/o insert (bp) 5428
  • Vector type
    Frog Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    cofilin 1 (cfl1)
  • Alt name
    XAC1
  • Species
    X. laevis (frog)
  • Mutation
    S3E
  • Entrez Gene
    cfl1.L (a.k.a. XELAEV_18022280mg, cfl1, cfl1-a, cfl1-b, cofilin-1, xac1, xac2)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unspecified (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Xenopus ADF/cofilins also are not targeted by shRNAs used here and can be expressed as active cofilin in mammalaian cells.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    XAC1 S3E was a gift from James Bamburg (Addgene plasmid # 245947 ; http://n2t.net/addgene:245947 ; RRID:Addgene_245947)

  • For your References section:

    ADF/cofilin controls cell polarity during fibroblast migration. Dawe HR, Minamide LS, Bamburg JR, Cramer LP. Curr Biol. 2003 Feb 4;13(3):252-7. doi: 10.1016/s0960-9822(03)00040-x. 10.1016/s0960-9822(03)00040-x PubMed 12573223
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