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LIMK1 T508EE
(Plasmid #245950)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 245950 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone manufacturer
    Unknown
  • Backbone size w/o insert (bp) 5428
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    LIM domain kinase 1
  • Alt name
    Limk1
  • Species
    M. musculus (mouse)
  • Mutation
    T508EE (insert of 2 glutamates)
  • Entrez Gene
    Limk1 (a.k.a. KIZ-1, LIMK-1, Limk)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unspecified (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Constitutively active LIMK1 increases the non-actin-binding phospho-cofilin in cells. Phospho-Ser3-cofilin is an activator of phospholipase D1.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    LIMK1 T508EE was a gift from Gordon Gill (Addgene plasmid # 245950 ; http://n2t.net/addgene:245950 ; RRID:Addgene_245950)

  • For your References section:

    Structural features of LIM kinase that control effects on the actin cytoskeleton. Edwards DC, Gill GN. J Biol Chem. 1999 Apr 16;274(16):11352-61. doi: 10.1074/jbc.274.16.11352. 10.1074/jbc.274.16.11352 PubMed 10196227
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