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ADF shRNA mouse
(Plasmid #245952)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 245952 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pBluescript
  • Backbone manufacturer
    Unknown
  • Backbone size w/o insert (bp) 2961
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    actin depolymerizing factor (ADF)
  • Alt name
    destrin
  • gRNA/shRNA sequence
    AAGTGATTGCAATCCGTGTAT
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Dstn (a.k.a. 2610043P17Rik, AD, ADF, AU042046, Dsn, co, corn1, sid, sid23p)
  • Promoter pol III H1

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unspecified (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Silences mouse ADF but not cofilin

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    ADF shRNA mouse was a gift from James Bamburg (Addgene plasmid # 245952 ; http://n2t.net/addgene:245952 ; RRID:Addgene_245952)
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