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SSH-3 WT
(Plasmid #245957)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 245957 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pShuttle CMV
  • Backbone manufacturer
    Gift from Bert Vogelstein in 1999
  • Backbone size w/o insert (bp) 7470
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Slingshot protein phosphatase 3
  • Alt name
    SSH-3L
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Ssh3 (a.k.a. SSH, SSH-3, SSH-3L)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unspecified (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This plasmid was created in the lab of Dr. Kensaku Mizuno (retired) in Tohoku University.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    SSH-3 WT was a gift from James Bamburg & Kensaku Mizuno & Kazumasa Ohashi (Addgene plasmid # 245957 ; http://n2t.net/addgene:245957 ; RRID:Addgene_245957)

  • For your References section:

    Differential activities, subcellular distribution and tissue expression patterns of three members of Slingshot family phosphatases that dephosphorylate cofilin. Ohta Y, Kousaka K, Nagata-Ohashi K, Ohashi K, Muramoto A, Shima Y, Niwa R, Uemura T, Mizuno K. Genes Cells. 2003 Oct;8(10):811-24. doi: 10.1046/j.1365-2443.2003.00678.x. 10.1046/j.1365-2443.2003.00678.x PubMed 14531860
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