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huCof C39A,C147A-mRFP
(Plasmid #245972)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 245972 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pShuttle CMV
  • Backbone manufacturer
    Gift from Bert Vogelstein in 1999
  • Backbone size w/o insert (bp) 7470
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    cofilin 1 (cfl1)
  • Species
    H. sapiens (human)
  • Mutation
    C39A,C147A
  • Entrez Gene
    CFL1 (a.k.a. CFL, HEL-S-15, cofilin)
  • Promoter CMV
  • Tag / Fusion Protein
    • mRFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unspecified (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Two cysteines modified here form a disulfide bond in cofilactin bundles isolated from stressed neurons.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    huCof C39A,C147A-mRFP was a gift from James Bamburg (Addgene plasmid # 245972 ; http://n2t.net/addgene:245972 ; RRID:Addgene_245972)

  • For your References section:

    Incorporation of cofilin into rods depends on disulfide intermolecular bonds: implications for actin regulation and neurodegenerative disease. Bernstein BW, Shaw AE, Minamide LS, Pak CW, Bamburg JR. J Neurosci. 2012 May 9;32(19):6670-81. doi: 10.1523/JNEUROSCI.6020-11.2012. 10.1523/JNEUROSCI.6020-11.2012 PubMed 22573689
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