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huCof WT-mRFP
(Plasmid #245973)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 245973 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pShuttle NSE
  • Backbone size w/o insert (bp) 7981
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    cofilin 1 (cfl1)
  • Species
    H. sapiens (human)
  • Entrez Gene
    CFL1 (a.k.a. CFL, HEL-S-15, cofilin)
  • Promoter NSE
  • Tag / Fusion Protein
    • mRFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unspecified (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Neuronal specific expression of mRFP-tagged WT cofilin

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    huCof WT-mRFP was a gift from James Bamburg (Addgene plasmid # 245973 ; http://n2t.net/addgene:245973 ; RRID:Addgene_245973)

  • For your References section:

    Growth cone-like waves transport actin and promote axonogenesis and neurite branching. Flynn KC, Pak CW, Shaw AE, Bradke F, Bamburg JR. Dev Neurobiol. 2009 Oct;69(12):761-79. doi: 10.1002/dneu.20734. 10.1002/dneu.20734 PubMed 19513994
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