pBOB-Mrap2-P2A-Mc4r-HA
(Plasmid
#245991)
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PurposeStimultaneously express HA C-terminal tagged mouse MC4R and untagged MRAP2 protein using a P2A linker, transiently via transfection or stably via lentivirus-mediated infection in mammalian cells.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 245991 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 |
Backbone
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Vector backbonepBOB
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Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer AGCTCGTTTAGTGAACCGTCAGATCG
- 3′ sequencing primer TACTCACCCCAACAGCTGGC (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBOB-Mrap2-P2A-Mc4r-HA was a gift from Zhao Zhang (Addgene plasmid # 245991 ; http://n2t.net/addgene:245991 ; RRID:Addgene_245991) -
For your References section:
GPR45 modulates Galpha(s) at primary cilia of the paraventricular hypothalamus to control food intake. Xun Y, Jiang Y, Xu B, Tang M, Ludwig S, Nakamura K, Mukhopadhyay S, Liu C, Beutler B, Zhang Z. Science. 2025 Jun 5;388(6751):eadp3989. doi: 10.1126/science.adp3989. Epub 2025 Jun 5. 10.1126/science.adp3989 PubMed 40472089