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pBOB-Gpr45-161-3xFlag
(Plasmid #245993)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 245993 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pBOB
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Gpr45-GPR161161 fusion
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1587
  • Mutation
    aa 1 to 327 of Gpr45; aa 357 to 558 of Gpr161
  • Entrez Gene
    Gpr161 (a.k.a. Gm208, Gm208Gpr, vl)
  • Entrez Gene
    Gpr45 (a.k.a. 9230112G11Rik, PSP24-1, PSP24-alpha)
  • Tag / Fusion Protein
    • 3xFlag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer AGCTCGTTTAGTGAACCGTCAGATCG
  • 3′ sequencing primer TACTCACCCCAACAGCTGGC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBOB-Gpr45-161-3xFlag was a gift from Zhao Zhang (Addgene plasmid # 245993 ; http://n2t.net/addgene:245993 ; RRID:Addgene_245993)

  • For your References section:

    GPR45 modulates Galpha(s) at primary cilia of the paraventricular hypothalamus to control food intake. Xun Y, Jiang Y, Xu B, Tang M, Ludwig S, Nakamura K, Mukhopadhyay S, Liu C, Beutler B, Zhang Z. Science. 2025 Jun 5;388(6751):eadp3989. doi: 10.1126/science.adp3989. Epub 2025 Jun 5. 10.1126/science.adp3989 PubMed 40472089
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