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pCMV-HA-Gnao1-B
(Plasmid #245999)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 245999 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pCMV
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Gnao1
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1065
  • GenBank ID
    NM_001113384
  • Entrez Gene
    Gnao1 (a.k.a. Galphao, Gnao, Hg1g, alphaO)
  • Tag / Fusion Protein
    • HA (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer TCTAAAAGCTGCGGAATTGT
  • 3′ sequencing primer GTGGTTTGTCCAAACTCATC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV-HA-Gnao1-B was a gift from Zhao Zhang (Addgene plasmid # 245999 ; http://n2t.net/addgene:245999 ; RRID:Addgene_245999)

  • For your References section:

    GPR45 modulates Galpha(s) at primary cilia of the paraventricular hypothalamus to control food intake. Xun Y, Jiang Y, Xu B, Tang M, Ludwig S, Nakamura K, Mukhopadhyay S, Liu C, Beutler B, Zhang Z. Science. 2025 Jun 5;388(6751):eadp3989. doi: 10.1126/science.adp3989. Epub 2025 Jun 5. 10.1126/science.adp3989 PubMed 40472089
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