dCas9-tra135
(Plasmid #246023)

• Purpose: Expresses dCas9 under control of Hsp70 promoter and gRNAs targeting Lucilia cuprina transformer gene
• Depositing Lab: Max Scott
• Publication: bioRxiv -- Conditional expression of Cas9 and dCas9 in Lucilia cuprina reveals dCas9-associated lethality (unpublished)

Backbone

• Vector backbone: pEF1
• Vector type: CRISPR

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: dCas9
• Species: Synthetic; Streptococcus pyogenes
• Insert Size (bp): 4101
• Tags / Fusion Proteins:
  • NLS (N terminal on insert)
  • NLS (C terminal on insert)

Gene/Insert 2

• Gene/Insert name: RFPex
• Insert Size (bp): 678

Resource Information

• Supplemental Documents:
  • dCas9 1-3-5 FINAL SEQ.gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • piggyBac Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://doi.org/10.1101/2025.08.12.669956 for bioRxiv preprint.
The depositor confirms that discrepancies between the depositor's sequence and Addgene's sequences have no functional consequences. Addgene NGS finds a 226 bp deletion that removes x6 TetOs upstream of the Hsp70 promoter compared to the annotated reference sequence.