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pDest_Tol2-gata2-hP1P2-eGFP
(Plasmid #246039)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 246039 Standard format: Plasmid sent in bacteria as agar stab 1 $89

Backbone

  • Vector backbone
    pDest
  • Total vector size (bp) 9972
  • Vector type
    Zebrafish Tol2 transgenesis for enhancer reporter assay

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    Human Peak1-Peak2 from EC1.45 enhancer cluster
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1616

Cloning Information for Gene/Insert 1

  • Cloning method Gateway Cloning
  • 5′ sequencing primer gata2_seq_Rev CCTACTGCTCCACATCCAGA
  • 3′ sequencing primer Peak1_seq_For CTGCTCCTTCTTCCAGGC; Peak1_seq_For2 GATTGAGAACAGGTGCAGC
  • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    gata2 promoter
  • Species
    D. rerio (zebrafish)
  • Insert Size (bp)
    1030

Gene/Insert 3

  • Gene/Insert name
    eGFP
  • Species
    Synthetic
  • Insert Size (bp)
    720

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDest_Tol2-gata2-hP1P2-eGFP was a gift from Hannah Long (Addgene plasmid # 246039)
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