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H1_sgRNA(GRIN2B)_SauCas9_AcrIIA5-AsLOV2(D41)
(Plasmid #246055)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 246055 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pUC19
  • Vector type
    Mammalian Expression, CRISPR, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SauCas9, sgRNA(GRIN2B) and AcrIIA5_AsLOV2-D41
  • Species
    Synthetic

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (unknown if destroyed)
  • 3′ cloning site BamHI (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    H1_sgRNA(GRIN2B)_SauCas9_AcrIIA5-AsLOV2(D41) was a gift from Dominik Niopek (Addgene plasmid # 246055 ; http://n2t.net/addgene:246055 ; RRID:Addgene_246055)

  • For your References section:

    A versatile anti-CRISPR platform for opto- and chemogenetic control of CRISPR-Cas9 and Cas12 across a wide range of orthologs. Brenker L, Aschenbrenner S, Bubeck F, Staykov K, Gebhardt C, Wolf B, Jendrusch M, Kroll AS, Becker J, Ambiel I, Fackler OT, Grimm D, Mathony J, Niopek D. Nucleic Acids Res. 2025 Jul 19;53(14):gkaf752. doi: 10.1093/nar/gkaf752. 10.1093/nar/gkaf752 PubMed 40794863
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