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ED_p_060
(Plasmid #246070)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 246070 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pET18
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB 10-beta
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Minimal CRISPR polymerase
  • Species
    E. coli
  • Promoter T7

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2025.03.28.646030 for the bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    ED_p_060 was a gift from Jennifer Doudna (Addgene plasmid # 246070 ; http://n2t.net/addgene:246070 ; RRID:Addgene_246070)

  • For your References section:

    A miniature CRISPR-Cas10 enzyme confers immunity by an inverse signaling pathway. Doherty EE, Adler BA, Yoon PH, Hsieh K, Loi K, Armbuster EG, Lahiri A, Bolling CS, Wilcox XE, Akkati A, Iavarone AT, Pogliano J, Doudna JA. bioRxiv [Preprint]. 2025 Apr 1:2025.03.28.646030. doi: 10.1101/2025.03.28.646030. 10.1101/2025.03.28.646030 PubMed 40196485
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