pCAMBIA1302-Pro35S:Fd-Irp9
              
              
                (Plasmid
                
                #246318)
              
            
            
            
          - 
            PurposeOverexpression of a bacterial bifunctional salicylate synthase, Irp9, targeted to the chloroplast
- 
              Depositing Lab
- 
          Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 246318 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
- 
            Vector backbonepCAMBIA1302
- 
              Vector typePlant Expression
Growth in Bacteria
- 
            Bacterial Resistance(s)Kanamycin, 50 μg/mL
- 
            Growth Temperature37°C
- 
            Growth Strain(s)DH5alpha
- 
            Copy numberHigh Copy
Gene/Insert
- 
                Gene/Insert nameFd-Irp9
- 
                    SpeciesYersinia enterocolitica
- Promoter cauliflower mosaic virus 35S
Cloning Information
- Cloning method Gibson Cloning
Resource Information
- 
            
            
            Supplemental Documents
Terms and Licenses
- 
        Academic/Nonprofit Terms
- 
      Industry Terms- Not Available to Industry
 
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
- 
              For your Materials & Methods section: pCAMBIA1302-Pro35S:Fd-Irp9 was a gift from Chung-Jui Tsai (Addgene plasmid # 246318 ; http://n2t.net/addgene:246318 ; RRID:Addgene_246318)
- 
                For your References section: Constitutively elevated salicylic acid levels alter photosynthesis and oxidative state but not growth in transgenic populus. Xue LJ, Guo W, Yuan Y, Anino EO, Nyamdari B, Wilson MC, Frost CJ, Chen HY, Babst BA, Harding SA, Tsai CJ. Plant Cell. 2013 Jul;25(7):2714-30. doi: 10.1105/tpc.113.112839. Epub 2013 Jul 31. 10.1105/tpc.113.112839 PubMed 23903318
 
    
 
                         
             
            