p201N.A2.COR6.6A.flag
(Plasmid
#246322)
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PurposeOverexpression of a cold-regulated gene, AtCOR6.6
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 246322 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonep201N-Cas9
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Vector typePlant Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCOR6.6-Flag
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SpeciesA. thaliana (mustard weed)
- Promoter Arabidopsis actin2 promoter
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Tag
/ Fusion Protein
- Flag (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
p201N.A2.COR6.6A.flag was a gift from Chung-Jui Tsai (Addgene plasmid # 246322 ; http://n2t.net/addgene:246322 ; RRID:Addgene_246322) -
For your References section:
Altering cold-regulated gene expression decouples the salicylic acid-growth trade-off in Arabidopsis. Ortega MA, Celoy RM, Chacon F, Yuan Y, Xue LJ, Pandey SP, Drowns MR, Kvitko BH, Tsai CJ. Plant Cell. 2024 Oct 3;36(10):4293-4308. doi: 10.1093/plcell/koae210. 10.1093/plcell/koae210 PubMed 39056470