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pIVT-PuroR-P2A-iCre-polyA_pKG03717
(Plasmid #246369)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 246369 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pENTR4
  • Backbone size w/o insert (bp) 2412
  • Vector type
    Mammalian Expression, Synthetic Biology ; in vitro transcription
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    PuroR-P2A-iCre
  • Species
    Synthetic
  • Insert Size (bp)
    1716
  • Mutation
    none

Cloning Information

  • Cloning method Golden Gate

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Also contains a puromycin gene . Please visit https://doi.org/10.1101/2024.06.19.599813 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pIVT-PuroR-P2A-iCre-polyA_pKG03717 was a gift from Kate Galloway (Addgene plasmid # 246369 ; http://n2t.net/addgene:246369 ; RRID:Addgene_246369)

  • For your References section:

    Programmable promoter editing for precise control of transgene expression. Kabaria SR, Bae Y, Ehmann ME, Lende-Dorn BA, Beitz AM, Peterman EL, Love KS, Ploessl DS, Galloway KE. Nat Biotechnol. 2025 Oct 13. doi: 10.1038/s41587-025-02854-y. 10.1038/s41587-025-02854-y PubMed 41083901
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