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pIVT-mRuby2-P2A-PuroR_pKG02755
(Plasmid #246371)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 246371 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pUC19
  • Backbone size w/o insert (bp) 3630
  • Vector type
    Mammalian Expression, Synthetic Biology ; in vitro transcription
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    mRuby2-P2A-PuroR
  • Species
    Synthetic
  • Insert Size (bp)
    1377
  • Mutation
    none

Cloning Information

  • Cloning method Golden Gate

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

PCR template for generating linear template for in vitro transcription. 5' and 3' beta globin UTR sequences were based on previous literature for effective modRNA expression in hiPSCs: 10.1016/j.crmeth.2022.100290The primers used for generating PCR template also came from this work, and are as follows:FWD: 5'- AGCTATAATACGACTCACTATAAGctcctgggcaacgtgctg-3', which encodes the T7 promoter compatible with AG CleanCap reagent (upper-case bases) and binds to the 5' UTR (lower-case bases)REV: 5'-poly(T)116-GCAATGAAAATAAATGTTTTTTATTAGGCAGAAT-3'}, which encodes the poly(A) tail and binds to the 3'-UTR.

Please visit https://doi.org/10.1101/2024.06.19.599813 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pIVT-mRuby2-P2A-PuroR_pKG02755 was a gift from Kate Galloway (Addgene plasmid # 246371 ; http://n2t.net/addgene:246371 ; RRID:Addgene_246371)

  • For your References section:

    Programmable promoter editing for precise control of transgene expression. Kabaria SR, Bae Y, Ehmann ME, Lende-Dorn BA, Beitz AM, Peterman EL, Love KS, Ploessl DS, Galloway KE. Nat Biotechnol. 2025 Oct 13. doi: 10.1038/s41587-025-02854-y. 10.1038/s41587-025-02854-y PubMed 41083901
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