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CMV-rtTA-BG-CMV-PuroR-BGH_pKG1701
(Plasmid #246376)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 246376 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    PiggyBac
  • Vector type
    Mammalian Expression, Synthetic Biology
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    rtTA
  • Species
    Other
  • Mutation
    none

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Also contains separately transcribed puromycin resistance . Please visit https://doi.org/10.1101/2024.06.19.599813 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CMV-rtTA-BG-CMV-PuroR-BGH_pKG1701 was a gift from Kate Galloway (Addgene plasmid # 246376 ; http://n2t.net/addgene:246376 ; RRID:Addgene_246376)

  • For your References section:

    Programmable promoter editing for precise control of transgene expression. Kabaria SR, Bae Y, Ehmann ME, Lende-Dorn BA, Beitz AM, Peterman EL, Love KS, Ploessl DS, Galloway KE. Nat Biotechnol. 2025 Oct 13. doi: 10.1038/s41587-025-02854-y. 10.1038/s41587-025-02854-y PubMed 41083901
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