pTR01F/hFGFR3c-N540K-V5
(Plasmid #246390)

• Purpose: for PiggyBac mediated integration and stable expression of hFGFR3c-N540K protein, mutant variant causes Hypochondroplasia and Thanatophoric dysplasia
• Depositing Lab: Pavel Krejčí
• Publication: Krejci Lab plasmids (unpublished)

Backbone

• Vector backbone: TR01F
• Vector type: Mammalian Expression
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: human FGFR3c-N540K
• Species: H. sapiens (human)
• Mutation: N540K substitution
• Entrez Gene: FGFR3 (a.k.a. ACH, CD333, CEK2, HSFGFR3EX, JTK4)
• Promoter: truncated CMV promoter
• Tag / Fusion Protein:
  • V5/His (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Unknown (unknown if destroyed)

Resource Information

• A portion of this plasmid was derived from a plasmid made by: The TR01F plasmid was obtained as a gift from Valeri Mossine (PMID: 24376882)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • piggyBac Limited Use Label License
• Industry Terms:
  • Not Available to Industry