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pUC19_PHF1_HTC3xF_Puro_donor
(Plasmid #246395)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 246395 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pUC19
  • Vector type
    Mammalian Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PHF1
  • Species
    H. sapiens (human)
  • Entrez Gene
    PHF1 (a.k.a. MTF2L2, PCL1, PHF2, TDRD19C, hPHF1)
  • Tag / Fusion Protein
    • HaloTag_3XFLAG (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUC19_PHF1_HTC3xF_Puro_donor was a gift from Yicheng Long (Addgene plasmid # 246395 ; http://n2t.net/addgene:246395 ; RRID:Addgene_246395)

  • For your References section:

    Distinct specificity and functions of PRC2 subcomplexes in human stem cells and cardiac differentiation. Much C, Rajkumar SM, Chen L, Cohen JM, Gade AR, Pitt GS, Long Y. Mol Cell. 2025 Aug 7:S1097-2765(25)00615-X. doi: 10.1016/j.molcel.2025.07.014. 10.1016/j.molcel.2025.07.014 PubMed 40803325
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