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yEPAC-R279L pDRF1-GW
(Plasmid #246454)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 246454 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pDRF1-GW
  • Vector type
    Yeast Expression
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    yEPAC-R269L
  • Species
    Synthetic
  • Insert Size (bp)
    4335
  • Promoter PMA1

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer AACAATCGTTAATAATTAATTAATTGG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Non-responsive yEPAC sensor, which itself can be found at https://www.addgene.org/191449/ (named Epac-SH225).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    yEPAC-R279L pDRF1-GW was a gift from Bas Teusink (Addgene plasmid # 246454 ; http://n2t.net/addgene:246454 ; RRID:Addgene_246454)

  • For your References section:

    A yeast FRET biosensor enlightens cAMP signaling. Botman D, O'Toole TG, Goedhart J, Bruggeman FJ, van Heerden JH, Teusink B. Mol Biol Cell. 2021 Jun 15;32(13):1229-1240. doi: 10.1091/mbc.E20-05-0319. Epub 2021 Apr 21. 10.1091/mbc.E20-05-0319 PubMed 33881352
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