pSRG100 (germline optimized 3xGCN4 for C. elegans)
(Plasmid
#246457)
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PurposeEncodes germline optimized 3xGCN4 for C. elegans. Useful to make CRISPR/Cas9 repair templates for 3xGCN4 integration in a gene of interest. For SunTag live-imaging of translation or protein labelling.
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 246457 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepBSK
- Total vector size (bp) 3096
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Vector typeWorm Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert name3xGCN4 (GLO)
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Alt name3x Suntag epitopes (germline optimized)
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Insert Size (bp)211
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made by3xGCN4 based on Addgene plasmid #61058.
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSRG100 (germline optimized 3xGCN4 for C. elegans) was a gift from Suzan Ruijtenberg (Addgene plasmid # 246457 ; http://n2t.net/addgene:246457 ; RRID:Addgene_246457)