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pCAGGS-GFP-UBA7-K709Sfs-IRES-mcherry
(Plasmid #246477)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 246477 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pCAGGS puro
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    AcGFP-UBA7-K709S*fs-IRES-mcherry
  • Alt name
    UBE1L
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2259
  • Mutation
    K709Sfs*
  • Entrez Gene
    UBA7 (a.k.a. D8, UBA1B, UBE1L, UBE2, UBE7)
  • Tag / Fusion Protein
    • AcGFP1 (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCAGGS-GFP-UBA7-K709Sfs-IRES-mcherry was a gift from Francisco Bustos (Addgene plasmid # 246477 ; http://n2t.net/addgene:246477 ; RRID:Addgene_246477)

  • For your References section:

    ISGylation is disrupted by UBA7 gene variants identified in individuals with neurodevelopmental disorder phenotypes. Bandi V, Venema M, Wallace I, Mol MO, Nikoncuk A, Schot R, van Slegtenhorst M, Bijlsma E, Khan A, White SM, Rius R, Delatycki MB, Narayanan V, Swatek KN, Barakat TS, Bustos F. iScience 2026. doi: 10.1016/j.isci.2026.115454 10.1016/j.isci.2026.115454
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