Skip to main content
Addgene
Open Menu

pET-p120RasGAP-581-1047-WT
(Plasmid #246487)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 246487 Standard format: Plasmid sent in bacteria as agar stab 1 $89
Add to Cart

Backbone

  • Vector backbone
    pET
  • Backbone size w/o insert (bp) 5403
  • Total vector size (bp) 6712
  • Modifications to backbone
    added TEV cleavage site
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    RASA1
  • Alt name
    p120RasGAP
  • Alt name
    RasGAP
  • Alt name
    p120GAP
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1401
  • Mutation
    deleted amino acids 1-580
  • GenBank ID
    NM_002890.3
  • Entrez Gene
    RASA1 (a.k.a. CM-AVM, CMAVM, CMAVM1, GAP, PKWS, RASA, RASGAP, p120, p120GAP, p120RASGAP)
  • Promoter T7lac
  • Tag / Fusion Protein
    • 6His (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7 terminal
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET-p120RasGAP-581-1047-WT was a gift from Titus Boggon (Addgene plasmid # 246487 ; http://n2t.net/addgene:246487 ; RRID:Addgene_246487)
Related items: