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pMAPLe4 PCMTD1
(Plasmid #246503)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 246503 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pMAPLe4
  • Backbone size w/o insert (bp) 6220
  • Total vector size (bp) 8473
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Use BL21(DE3) for expression
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Protein-L-isoaspartate O-methyltransferase domain-containing protein 1
  • Alt name
    PCMTD1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2253
  • Mutation
    Residue 312 is Isoleucine in this plasmid but occurs naturally in humans as either an Asparagine or Isoleucine polymorphism. Uniprot Q96MG8-1 reflects the Asparagine polymorphism.
  • Entrez Gene
    PCMTD1
  • Tag / Fusion Protein
    • His-tag (N terminal on insert)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Induce with 0.5 mM IPTG at OD600 = 0.5 - 0.7 and express for 3 hours at 37°C after induction

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMAPLe4 PCMTD1 was a gift from Steven Clarke (Addgene plasmid # 246503 ; http://n2t.net/addgene:246503 ; RRID:Addgene_246503)

  • For your References section:

    Human Protein-l-isoaspartate O-Methyltransferase Domain-Containing Protein 1 (PCMTD1) Associates with Cullin-RING Ligase Proteins. Warmack RA, Pang EZ, Peluso E, Lowenson JD, Ong JY, Torres JZ, Clarke SG. Biochemistry. 2022 May 17;61(10):879-894. doi: 10.1021/acs.biochem.2c00130. Epub 2022 Apr 29. 10.1021/acs.biochem.2c00130 PubMed 35486881
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