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pMAPLe4 PCMTD1 T62 H67 M68
(Plasmid #246506)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 246506 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pMAPLe4
  • Backbone size w/o insert (bp) 6220
  • Total vector size (bp) 8473
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Use BL21(DE3) for expression
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Protein-L-isoaspartate O-methyltransferase domain-containing protein 1
  • Alt name
    PCMTD1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2253
  • Mutation
    Residue 312 is Isoleucine in this plasmid but occurs naturally in humans as either an Asparagine or Isoleucine polymorphism. Uniprot Q96MG8-1 reflects the Asparagine polymorphism. Changed Histidine 62 to Threonine, Cysteine 67 to Histidine, and Isoleucine 68 to Methionine.
  • Entrez Gene
    PCMTD1
  • Tag / Fusion Protein
    • His-tag (N terminal on insert)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Produced for us in this study by Twist Bioscience using our order specifications

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Induce with 0.5 mM IPTG at OD600 = 0.5 - 0.7 and express for 3 hours at 37°C after induction.

Please visit https://doi.org/10.1101/2025.05.21.654933 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMAPLe4 PCMTD1 T62 H67 M68 was a gift from Steven Clarke (Addgene plasmid # 246506 ; http://n2t.net/addgene:246506 ; RRID:Addgene_246506)

  • For your References section:

    Structural basis for L-isoaspartyl-containing protein recognition by the PCMTD1 cullin-RING E3 ubiquitin ligase. Pang EZ, Zhao B, Flowers C, Oroudjeva E, Winter JB, Pandey V, Sawaya MR, Wohlschlegel J, Loo JA, Rodriguez JA, Clarke SG. bioRxiv [Preprint]. 2025 May 21:2025.05.21.654933. doi: 10.1101/2025.05.21.654933. 10.1101/2025.05.21.654933 PubMed 40475564
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