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CBE-Cas12a-GFP
(Plasmid #246533)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 246533 Standard format: Plasmid sent in bacteria as agar stab 1 $89

Backbone

  • Vector backbone
    CBE-Cas12a-GFP
  • Vector type
    Plant Expression, CRISPR
  • Promoter CmYLCV
  • Selectable markers
    Chlorsulfuron

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Cloning Information

  • Cloning method Golden Gate

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CBE-Cas12a-GFP was a gift from Nian Wang (Addgene plasmid # 246533 ; http://n2t.net/addgene:246533 ; RRID:Addgene_246533)
  • For your References section:

    Transgene-free genome editing of vegetatively propagated and perennial plant species in the T0 generation via a co-editing strategy. Huang X, Jia H, Xu J, Wang Y, Wen J, Wang N. Nat Plants. 2023 Oct;9(10):1591-1597. doi: 10.1038/s41477-023-01520-y. Epub 2023 Sep 18. 10.1038/s41477-023-01520-y PubMed 37723203