CBE-Cas12a-GFP
              
              
                (Plasmid
                
                #246533)
              
            
            
            
          - 
            Purpose(Empty Backbone) Plant co-editing vector
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              Depositing Lab
 - 
          Sequence Information
 
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 246533 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
- 
            Vector backboneCBE-Cas12a-GFP
 - 
              Vector typePlant Expression, CRISPR
 - Promoter CmYLCV
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                Selectable markersChlorsulfuron
 
Growth in Bacteria
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            Bacterial Resistance(s)Kanamycin, 50 μg/mL
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            Growth Temperature37°C
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            Growth Strain(s)DH5alpha
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            Copy numberUnknown
 
Cloning Information
- Cloning method Golden Gate
 
Terms and Licenses
- 
        Academic/Nonprofit Terms
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      Industry Terms
- Not Available to Industry
 
 
Trademarks:
- Zeocin® is an InvivoGen trademark.
 
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
CBE-Cas12a-GFP was a gift from Nian Wang (Addgene plasmid # 246533 ; http://n2t.net/addgene:246533 ; RRID:Addgene_246533) - 
                
For your References section:
Transgene-free genome editing of vegetatively propagated and perennial plant species in the T0 generation via a co-editing strategy. Huang X, Jia H, Xu J, Wang Y, Wen J, Wang N. Nat Plants. 2023 Oct;9(10):1591-1597. doi: 10.1038/s41477-023-01520-y. Epub 2023 Sep 18. 10.1038/s41477-023-01520-y PubMed 37723203