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pTW334e2
(Plasmid #246586)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 246586 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    cloDF13
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    AtRaf1, AtRaf2, AtRbcX2, AtCpn60alpha, AtCpn60beta, AtCpn20
  • Species
    A. thaliana (mustard weed)
  • Entrez Gene
    AT3G04550 (a.k.a. AT3G04550, F7O18.2, F7O18_2)
  • Entrez Gene
    CPN60B (a.k.a. AT1G55490, CPN60BETA, Cpn60beta1, LEN1, LESION INITIATION 1, T5A14.11, T5A14_11, chaperonin 60 beta, chaperonin-60beta1)
  • Entrez Gene
    CPN60A (a.k.a. AT2G28000, CH-CPN60A, CHAPERONIN-60ALPHA, CHLOROPLAST CHAPERONIN 60ALPHA, Cpn60alpha1, SCHLEPPERLESS, SLP, T1E2.8, T1E2_8, chaperonin-60alpha, chaperonin-60alpha1)
  • Entrez Gene
    CPN20 (a.k.a. AT5G20720, ATCPN21, CHAPERONIN 10, CHCPN10, CHLOROPLAST CHAPERONIN 10, CPN10, CPN21, T1M15.120, T1M15_120, chaperonin 20)
  • Entrez Gene
    RbcX2 (a.k.a. AT5G19855, AtRbcX2, homologue of cyanobacterial RbcX 2)

Cloning Information

  • Cloning method Golden Gate

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTW334e2 was a gift from Tina Wang (Addgene plasmid # 246586 ; http://n2t.net/addgene:246586 ; RRID:Addgene_246586)

  • For your References section:

    Directed evolution of a plant Rubisco chaperone with altered client recognition. Li S, Lee B, Lao Y, Lertwiriyapiti S, Huang X, Wang T. Proc Natl Acad Sci U S A. 2025 Sep 16;122(37):e2510701122. doi: 10.1073/pnas.2510701122. Epub 2025 Sep 11. 10.1073/pnas.2510701122 PubMed 40932770
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