CMV-TNFa-Zdk-HT
(Plasmid
#246613)
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PurposeExpresses RudLOV cargo(TNFα) in mammalian cells.
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 246613 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepEGFP-N1
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Backbone manufacturerClontech
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nametumor necrosis factor
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Alt nameTNF alpha
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SpeciesH. sapiens (human)
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Insert Size (bp)725
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Entrez GeneTNF (a.k.a. DIF, IMD127, TNF-alpha, TNFA, TNFSF2, TNLG1F)
- Promoter CMV
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Tags
/ Fusion Proteins
- Zdk1 (C terminal on insert)
- Halotag7 (C terminal on insert)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer CMV Forward
- 3′ sequencing primer SV40pA-R
- (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
CMV-TNFa-Zdk-HT was a gift from Akiko Satoh (Addgene plasmid # 246613 ; http://n2t.net/addgene:246613 ; RRID:Addgene_246613) -
For your References section:
Studying Cargo Transport Using RudLOV. Tago T, Satoh T, Satoh AK. Bio Protoc. 2025 Oct 20;15(20):e5468. doi: 10.21769/BioProtoc.5468. eCollection 2025 Oct 20. 10.21769/BioProtoc.5468 PubMed 41141641