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AviTag-His6-OAZ-SPM(FrpA 298-318)-GSG-SpyTag003
(Plasmid #246647)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 246647 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pET28
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5369
  • Total vector size (bp) 5951
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    AviTag-His6-OAZ-SPM(FrpA 298-318)-GSG-SpyTag003
  • Species
    H. sapiens (human), Synthetic; Neisseria meningitidis serotype C (UniProt: P55126)
  • Insert Size (bp)
    582
  • Mutation
    Orinithine Decarboxylase Antizyme (OAZ) with C175A mutation to minimize disulfide-based aggregation, fused to the N-terminal split self-processing module (SPM; residues 298-318) of FrpA from Neisseria meningitidis serotype C (UniProt: P55126)
  • Promoter T7
  • Tags / Fusion Proteins
    • AviTag (N terminal on insert)
    • His-tag (N terminal on insert)
    • SpyTag003 (C terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    AviTag-His6-OAZ-SPM(FrpA 298-318)-GSG-SpyTag003 was a gift from Mark Howarth (Addgene plasmid # 246647 ; http://n2t.net/addgene:246647 ; RRID:Addgene_246647)

  • For your References section:

    Split NeissLock with Spy-Acceleration Arms Mammalian Proteins for Anhydride-Mediated Cell Ligation. Lim SYT, Keeble AH, Howarth MR. ACS Chem Biol. 2025 Sep 15. doi: 10.1021/acschembio.5c00515. 10.1021/acschembio.5c00515 PubMed 40947984
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