Skip to main content
Addgene
Open Menu

Neo-2/15-TGFa-SPMn-SpyTag003
(Plasmid #246650)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 246650 Standard format: Plasmid sent in bacteria as agar stab 1 $89
Add to Cart

Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5428
  • Total vector size (bp) 6100
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Neo-2/15-TGFa-SPMn-SpyTag003
  • Species
    H. sapiens (human), Synthetic; Neisseria meningitidis serotype C (UniProt: P55126)
  • Insert Size (bp)
    672
  • Mutation
    Construct contains the tPA signal peptide, Neo2/15, GSS linker, TGFα (residues 40–89 of human protransforming growth factor alpha), N-terminal fragment of the self-processing module of FrpA (SPMn 298-318), GSG linker, SpyTag003
  • Promoter CMV promoter
  • Tags / Fusion Proteins
    • Signal peptide (N terminal on insert)
    • SpyTag003 (C terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Neo-2/15-TGFa-SPMn-SpyTag003 was a gift from Mark Howarth (Addgene plasmid # 246650 ; http://n2t.net/addgene:246650 ; RRID:Addgene_246650)

  • For your References section:

    Split NeissLock with Spy-Acceleration Arms Mammalian Proteins for Anhydride-Mediated Cell Ligation. Lim SYT, Keeble AH, Howarth MR. ACS Chem Biol. 2025 Sep 15. doi: 10.1021/acschembio.5c00515. 10.1021/acschembio.5c00515 PubMed 40947984
Related items: