pDonor AVIL-P2A-iCre
(Plasmid
#246652)
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PurposeDonor plasmid for Crispr/Cas9 gene editing of the human AVIL locus
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 246652 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepBluescript
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Backbone manufacturerStratagene
- Backbone size w/o insert (bp) 2958
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Vector typeMammalian Expression, CRISPR
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature30°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameP2A-iCre flanked by AVIL homology regions
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SpeciesH. sapiens (human), Synthetic
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Entrez GeneAVIL (a.k.a. p92, DOC6, ADVIL, FLJ12386, MGC133244, DKFZp779O1812)
Cloning Information
- Cloning method Ligation Independent Cloning
Resource Information
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A portion of this plasmid was derived from a plasmid made byiCre; cloned from Addgene 51904: paavCAG-iCre, Druckmann et al Neuron. 2014 Feb 5;81(3):629-40.
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDonor AVIL-P2A-iCre was a gift from Joriene de Nooij (Addgene plasmid # 246652 ; http://n2t.net/addgene:246652 ; RRID:Addgene_246652) -
For your References section:
Derivation and analysis of human somatic sensory neuron subtypes facilitated through fluorescent hPSC reporters. Malka-Gibor E, Oliver KM, Chen S, Garcia-Diaz A, Williams D, Henry JA, Akcan BT, Corneo B, de Nooij JC. Stem Cell Reports. 2026 Jan 2:102753. doi: 10.1016/j.stemcr.2025.102753. 10.1016/j.stemcr.2025.102753 PubMed 41483810